PoplarV1, Main, Exploration, bibRecord, 004077

Cloning and molecular characterisation of a gene encoding cytochrome P450 C-23 steroid hydroxylase (CYP90) from aspen.

Identifieur interne : 004077 ( Main/Exploration ); précédent : 004076; suivant : 004078

Cloning and molecular characterisation of a gene encoding cytochrome P450 C-23 steroid hydroxylase (CYP90) from aspen.

Auteurs : Xin-Yu Wang [République populaire de Chine] ; Chong-Ying Wang ; Olof Olsson

Source :

Yi chuan xue bao = Acta genetica Sinica [ 0379-4172 ] ; 2005.

RBID : pubmed:16011030

Descripteurs français

English descriptors

KwdEn :
- Amino Acid Sequence (MeSH), Arabidopsis Proteins (genetics), Cloning, Molecular (MeSH), Cytochrome P-450 Enzyme System (genetics), DNA, Complementary (chemistry), DNA, Complementary (genetics), DNA, Plant (chemistry), DNA, Plant (genetics), Molecular Sequence Data (MeSH), Open Reading Frames (genetics), Phylogeny (MeSH), Plant Proteins (genetics), Populus (enzymology), Populus (genetics), Sequence Analysis, DNA (MeSH), Sequence Homology, Amino Acid (MeSH), Steroid Hydroxylases (genetics).
MESH :
- chemical , chemistry : DNA, Complementary, DNA, Plant.
- chemical , genetics : Arabidopsis Proteins, Cytochrome P-450 Enzyme System, DNA, Complementary, DNA, Plant, Plant Proteins, Steroid Hydroxylases.
- enzymology : Populus.
- genetics : Open Reading Frames, Populus.
- Amino Acid Sequence, Cloning, Molecular, Molecular Sequence Data, Phylogeny, Sequence Analysis, DNA, Sequence Homology, Amino Acid.

Abstract

Gene CPD (constitutive photomorphogenesis and dwarf) encodes a cytochrome P450 steroid side-chain hydroxylase (CYP90) involved in biosynthesis of brassinosteroids (BRs) in Arabidopsis thanalia. To dissect the molecular mechanism underlying the biosynthesis and action of the new phytohormone in perennial woody plants, a 1442 bp cDNA and a 1900 bp genomic DNA fragments whose 3'- half was well overlapped with the 5'-half of the former were obtained from a hybrid aspen (Populus tremula x tremudelois) by screening of the cDNA and genomic DNA libraries of this tree using a cDNA fragment from the Arabidopsis CPD gene in the present study. Sequence analysis revealed that the open reading frame comprising the cDNA and genomic DNA fragments encoded a protein of 476 amino acids with a molecular weight of 63 kD. This protein is four amino acid residues longer than Arabidopsis CYP90, and 78.32% identical to the latter. Also it shared high homologies with the Arabidopsis CYP90 in all known functional domains,including steroid-binding region closely related to BR biosynthesis, indicating a high conservatism of the putative CDP gene between annual herb and perennial woody plant species.

PubMed: 16011030

Affiliations:

République populaire de Chine

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Le document en format XML

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<author><name sortKey="Wang, Xin Yu" sort="Wang, Xin Yu" uniqKey="Wang X" first="Xin-Yu" last="Wang">Xin-Yu Wang</name>
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<term>Cytochrome P-450 Enzyme System (genetics)</term>
<term>DNA, Complementary (chemistry)</term>
<term>DNA, Complementary (genetics)</term>
<term>DNA, Plant (chemistry)</term>
<term>DNA, Plant (genetics)</term>
<term>Molecular Sequence Data (MeSH)</term>
<term>Open Reading Frames (genetics)</term>
<term>Phylogeny (MeSH)</term>
<term>Plant Proteins (genetics)</term>
<term>Populus (enzymology)</term>
<term>Populus (genetics)</term>
<term>Sequence Analysis, DNA (MeSH)</term>
<term>Sequence Homology, Amino Acid (MeSH)</term>
<term>Steroid Hydroxylases (genetics)</term>
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<term>ADN complémentaire (génétique)</term>
<term>ADN des plantes (composition chimique)</term>
<term>ADN des plantes (génétique)</term>
<term>Analyse de séquence d'ADN (MeSH)</term>
<term>Cadres ouverts de lecture (génétique)</term>
<term>Clonage moléculaire (MeSH)</term>
<term>Cytochrome P-450 enzyme system (génétique)</term>
<term>Données de séquences moléculaires (MeSH)</term>
<term>Phylogenèse (MeSH)</term>
<term>Populus (enzymologie)</term>
<term>Populus (génétique)</term>
<term>Protéines d'Arabidopsis (génétique)</term>
<term>Protéines végétales (génétique)</term>
<term>Similitude de séquences d'acides aminés (MeSH)</term>
<term>Steroid hydroxylases (génétique)</term>
<term>Séquence d'acides aminés (MeSH)</term>
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<keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>DNA, Complementary</term>
<term>DNA, Plant</term>
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<term>Populus</term>
<term>Protéines d'Arabidopsis</term>
<term>Protéines végétales</term>
<term>Steroid hydroxylases</term>
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<keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term>
<term>Cloning, Molecular</term>
<term>Molecular Sequence Data</term>
<term>Phylogeny</term>
<term>Sequence Analysis, DNA</term>
<term>Sequence Homology, Amino Acid</term>
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<keywords scheme="MESH" xml:lang="fr"><term>Analyse de séquence d'ADN</term>
<term>Clonage moléculaire</term>
<term>Données de séquences moléculaires</term>
<term>Phylogenèse</term>
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<front><div type="abstract" xml:lang="en">Gene CPD (constitutive photomorphogenesis and dwarf) encodes a cytochrome P450 steroid side-chain hydroxylase (CYP90) involved in biosynthesis of brassinosteroids (BRs) in Arabidopsis thanalia. To dissect the molecular mechanism underlying the biosynthesis and action of the new phytohormone in perennial woody plants, a 1442 bp cDNA and a 1900 bp genomic DNA fragments whose 3'- half was well overlapped with the 5'-half of the former were obtained from a hybrid aspen (Populus tremula x tremudelois) by screening of the cDNA and genomic DNA libraries of this tree using a cDNA fragment from the Arabidopsis CPD gene in the present study. Sequence analysis revealed that the open reading frame comprising the cDNA and genomic DNA fragments encoded a protein of 476 amino acids with a molecular weight of 63 kD. This protein is four amino acid residues longer than Arabidopsis CYP90, and 78.32% identical to the latter. Also it shared high homologies with the Arabidopsis CYP90 in all known functional domains,including steroid-binding region closely related to BR biosynthesis, indicating a high conservatism of the putative CDP gene between annual herb and perennial woody plant species.</div>
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<Abstract><AbstractText>Gene CPD (constitutive photomorphogenesis and dwarf) encodes a cytochrome P450 steroid side-chain hydroxylase (CYP90) involved in biosynthesis of brassinosteroids (BRs) in Arabidopsis thanalia. To dissect the molecular mechanism underlying the biosynthesis and action of the new phytohormone in perennial woody plants, a 1442 bp cDNA and a 1900 bp genomic DNA fragments whose 3'- half was well overlapped with the 5'-half of the former were obtained from a hybrid aspen (Populus tremula x tremudelois) by screening of the cDNA and genomic DNA libraries of this tree using a cDNA fragment from the Arabidopsis CPD gene in the present study. Sequence analysis revealed that the open reading frame comprising the cDNA and genomic DNA fragments encoded a protein of 476 amino acids with a molecular weight of 63 kD. This protein is four amino acid residues longer than Arabidopsis CYP90, and 78.32% identical to the latter. Also it shared high homologies with the Arabidopsis CYP90 in all known functional domains,including steroid-binding region closely related to BR biosynthesis, indicating a high conservatism of the putative CDP gene between annual herb and perennial woody plant species.</AbstractText>
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Cloning and molecular characterisation of a gene encoding cytochrome P450 C-23 steroid hydroxylase (CYP90) from aspen.

Cloning and molecular characterisation of a gene encoding cytochrome P450 C-23 steroid hydroxylase (CYP90) from aspen.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki